Publications

2012

  • Palmberger D, Wilson IB, Berger I, Grabherr R, Rendic D. (2012) SweetBac: a new approach for the production of mammalianised glycoproteins in insect cells. PLoS one. 2012;7(4).
  • Heinl S, Wibberg D, Eikmeyer F, Szczepanowski R, Blom J, Linke B, Goesmann A, Grabherr R, Schwab H, Pühler A, Schlüter A. (2012) Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage. J Biotechnol. [Epub ahead of print]
  • Ribeiro S, Mairhofer J, Madeira C, Diogo M, Lobato da Silva C, Monteiro G, Grabherr R, Cabral J (2012) Plasmid DNA size does affect non-viral gene delivery efficiency in stem cells. Cell Reprogram 2012, 14(2):130-7.
  • Baumann M, Höppner MP, Meier M, Pontiller J, Ernst W, Grabherr R, Mauceli E, Grabherr MG. (2012) Artificially designed promoters: understanding the role of spatial features and canonical binding sites in transcription. Bioeng Bugs, Epub.

2011

  • Spath K, Heinl S, Egger E, Grabherr R. (2011) Lactobacillus plantarum and Lactobacillus buchneri as Expression Systems: Evaluation of Different Origins of Replication for the Design of Suitable Shuttle Vectors. Mol Biotechnol, Epub.
  • Heinl S, Spath K, Egger E, Grabherr R. (2011) Sequence analysis and characterization of two cryptic plasmids derived from Lactobacillus buchneri CD034. Plasmid, 66(3):159-68.
  • Grabherr MG, Pontiller J, Mauceli E, Ernst W, Baumann M, Biagi T, Swofford R, Russell P, Zody MC, Di Palma F, Lindblad-Toh K, Grabherr R. (2011) Exploiting nucleotide composition to engineer promoters. PLoS One, 6(5).
  • Heinl, S., Hartinger, D., Thamhesl, M., Schatzmayr, G., Moll,W.-D., Grabherr R. (2011) An aminotransferase from bacterium ATCC 55552 deaminates hydrolyzed fumonisin B(1). Biodegradation, 22(1):25-30.
  • Palmberger D, Rendić D, Tauber P, Krammer F, Wilson IB, Grabherr R. (2010) Insect cells for antibody production: evaluation of an efficient alternative. J Biotechnol, 20;153(3-4):160-6.

2010

  • Hartinger D, Heinl S, Schwartz HE, Grabherr R, Schatzmayr G, Haltrich D, Moll WD. (2010) Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B(1). Microb Cell Fact, 9:62.
  • Krammer F, Grabherr R. (2010) Alternative influenza vaccines made by insect cells. Trends Mol Med, 16(7):313-20.
  • Krammer F, Pontiller J, Tauer C, Palmberger D, Maccani A, Baumann M, Grabherr R. (2010) Evaluation of the influenza A replicon for transient expression of recombinant proteins in mammalian cells. PLoS One, 5(10):e13265.
  • Baumann M, Pontiller J, Ernst W. (2010) Structure and basal transcription complex of RNA polymerase II core promoters in the mammalian genome: an overview. Mol Biotechnol, 45(3):241-7.
  • Pontiller J, Maccani A, Baumann M, Klancnik I, Ernst W. (2010) Identification of CHO endogenous gene regulatory elements. Mol Biotechnol, 45(3):235-40.
  • Krammer F, Schinko T, Palmberger D, Tauer C, Messner P, Grabherr R. (2010) Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines. Mol Biotechnol, 45(3):226-34.
  • Krammer F, Schinko T, Messner P, Palmberger D, Ferko B, Grabherr R. (2010) Influenza virus-like particles as an antigen-carrier platform for the ESAT-6 epitope of Mycobacterium tuberculosis. J Virol Methods, 167(1):17-22.
  • Grabherr R, Ernst W. (2010) Baculovirus for eukaryotic protein display. Curr Gene Ther. ,10(3):195-200.
  • Mairhofer J., Cserjan-Puschmann M., Striedner G., Nöbauer K., Razzazi-Fazeli E., Grabherr R. (2010) Marker-free plasmids for gene therapeutic applications - lack of antibiotic resistance gene substantially improves the manufacturing process. J Biotechnol, 146(3):130-7.
  • Krammer F., Nakowitsch S., Messner P., Palmberger D., Ferko B., Reingard R. (2010) Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice. Biotechnol J, 5, 17-23.

2009

  • Heinl S., Hartinger D., Thamhesl M., Vekiru E., Krska R., Schatzmayr G., Moll W.D., Grabherr, R (2009) Degradation of fumonisin B(1) by the consecutive action of two bacterial enzymes. J Biotechnol, 2, 120-129 
  • Mairhofer J., Roppert K., Ertl P. (2009) Microfluidic Systems for Pathogen Sensing: A Review. Sensors, 9(6), 4804-4823.

2008

  • Pontiller, J., Gross, S., Thaisuchat, H., Hesse, F. and Ernst, W. (2008) Identification of CHO Endogenous Promoter Elements Based on a Genomic Library Approach. Mol Biotechnol.
  • Mairhofer, J. and Grabherr, R. (2008) Rational Vector Design for Efficient Non-viral Gene Delivery: Challenges Facing the Use of Plasmid DNA. Mol Biotechnol.
  • Mairhofer, J., Pfaffenzeller, I., Merz, D. and Grabherr, R. (2008) A novel antibiotic free plasmid selection system: advances in safe and efficient DNA therapy. Biotechnol J, 3, 83-89.

2006

  • Fischlechner, M., Toellner, L., Messner, P., Grabherr, R. and Donath, E. (2006) Virus-engineered colloidal particles--a surface display system. Angew Chem Int Ed Engl, 45, 784-789.
  • Matilainen, H., Mäkelä, A., Riikonen, R., Saloniemi, T., Korhonen, E., Hyypiä, T., Heino, J., Grabherr, R. and Oker-Blom, C. (2006) RGD motifs on the surface of baculovirus enhance transduction of human lung carcinoma cells. J Biotechnol, 125, 114-126.
  • Ernst, W., Schinko, T., Spenger, A., Oker-Blom, C. and Grabherr, R. (2006) Improving baculovirus transduction of mammalian cells by surface display of a RGD-motif. J Biotechnol, 126, 237-240.
  • Toellner, L., Fischlechner, M., Ferko, B., Grabherr, R. and Donath, E. (2006) Virus-coated layer-by-layer colloids as a multiplex suspension array for the detection and quantification of virus-specific antibodies. Clin Chem, 52, 1575-1583.
  • Pfaffenzeller, I., Mairhofer, J., Striedner, G., Bayer, K. and Grabherr, R. (2006) Using ColE1-derived RNA I for suppression of a bacterially encoded gene: implication for a novel plasmid addiction system. Biotechnol J, 1, 675-681.
  • Nakowitsch, S., Kittel, C., Ernst, W., Egorov, A. and Grabherr, R. (2006) Optimization of baculovirus transduction on FreeStyle293 cells for the generation of influenza B/Lee/40. Mol Biotechnol, 34, 157-164.

2004

  • Ojala, K., Koski, J., Ernst, W., Grabherr, R., Jones, I. and Oker-Blom, C. (2004) Improved display of synthetic IgG-binding domains on the baculovirus surface. Technol Cancer Res Treat, 3, 77-84.
  • Spenger, A., Ernst, W., Condreay, J., Kost, T. and Grabherr, R. (2004) Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells. Protein Expr Purif, 38, 17-23.

2003

  • Poomputsa, K., Kittel, C., Egorov, A., Ernst, W. and Grabherr, R. (2003) Generation of recombinant influenza virus using baculovirus delivery vector. J Virol Methods, 110, 111-114.
  • Oker-Blom, C., Airenne, K. and Grabherr, R. (2003) Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery. Brief Funct Genomic Proteomic, 2, 244-253.

2002

  • Grabherr, R., Nilsson, E., Striedner, G. and Bayer, K. (2002) Stabilizing plasmid copy number to improve recombinant protein production. Biotechnol Bioeng, 77, 142-147.
  • Grabherr, R. and Bayer, K. (2002) Impact of targeted vector design on Co/E1 plasmid replication. Trends Biotechnol, 20, 257-260.
  • Spenger, A., Grabherr, R., Töllner, L., Katinger, H. and Ernst, W. (2002) Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64. Eur J Biochem, 269, 4458-4467.
  • Marzban, G., Grillari, J., Reisinger, E., Hemetsberger, T., Grabherr, R. and Katinger, H. (2002) Age-related alterations in the protein expression profile of C57BL/6J mouse pituitaries. Exp Gerontol, 37, 1451-1460.

2001

  • Grillari, J., Fortschegger, K., Grabherr, R., Hohenwarter, O., Kunert, R. and Katinger, H. (2001) Analysis of alterations in gene expression after amplification of recombinant genes in CHO cells. J Biotechnol, 87, 59-65.
  • Grabherr, R. and Ernst, W. (2001) The baculovirus expression system as a tool for generating diversity by viral surface display. Comb Chem High Throughput Screen, 4, 185-192.
  • Grabherr, R., Ernst, W., Oker-Blom, C. and Jones, I. (2001) Developments in the use of baculoviruses for the surface display of complex eukaryotic proteins. Trends Biotechnol, 19, 231-236.

2000

  • Grillari, J., Hohenwarter, O., Grabherr, R. and Katinger, H. (2000) Subtractive hybridization of mRNA from early passage and senescent endothelial cells. Exp Gerontol, 35, 187-197.
  • Ernst, W., Spenger, A., Toellner, L., Katinger, H. and Grabherr, R. (2000) Expanding baculovirus surface display. Modification of the native coat protein gp64 of Autographa californica NPV. Eur J Biochem, 267, 4033-4039.

1998

  • Ernst, W., Grabherr, R., Wegner, D., Borth, N., Grassauer, A. and Katinger, H. (1998) Baculovirus surface display: construction and screening of a eukaryotic epitope library. Nucleic Acids Res, 26, 1718-1723.

1997

  • Grabherr, R., Ernst, W., Doblhoff-Dier, O., Sara, M. and Katinger, H. (1997) Expression of foreign proteins on the surface of Autographa californica nuclear polyhedrosis virus. Biotechniques, 22, 730-735.

1996

  • Knezević, M., Vorauer-Uhl, K., Hohenwarter, O., Steindl, F., Grabherr, R. and Raspor, P. (1996) Human interleukin-2 (IL-2) expressed by transfected mammalian cells. Pflugers Arch, 431, R227-228.

1994

  • Ernst, W., Grabherr, R. and Katinger, H. (1994) Direct cloning into the Autographa californica nuclear polyhedrosis virus for generation of recombinant baculoviruses. Nucleic Acids Res, 22, 2855-2856.
  • Engelhardt, O., Grabherr, R., Himmler, G. and Rüker, F. (1994) Two-step cloning of antibody variable domains in a phage display vector. Biotechniques, 17, 44, 46.
  • Zhang, Y., Strasser, P., Grabherr, R. and Van Etten, J. (1994) Hairpin loop structure at the termini of the chlorella virus PBCV-1 genome. Virology, 202, 1079-1082.

1993

  • Nelson, M., Zhang, Y., Steffens, D., Grabherr, R. and Van Etten, J. (1993) Sequencing two DNA templates in five channels by digital compression. Proc Natl Acad Sci U S A, 90, 1647-1651.

1992

  • Nelson, M., Van Etten, J. and Grabherr, R. (1992) DNA sequencing of four bases using three lanes. Nucleic Acids Res, 20, 1345-1348.
  • Grabherr, R., Strasser, P. and Van Etten, J. (1992) The DNA polymerase gene from chlorella viruses PBCV-1 and NY-2A contains an intron with nuclear splicing sequences. Virology, 188, 721-731.

1990

  • Shields, S., Burbank, D., Grabherr, R. and van Etten, J. (1990) Cloning and sequencing the cytosine methyltransferase gene M. CviJI from Chlorella virus IL-3A. Virology, 176, 16-24.