Influenza Vaccine Design

Recent outbreaks of swine-origin pandemic H1N1 Influenza A (Schweinegrippe, pandemische H1N1 Influenza, neue Grippe) highlight the importance of rapid and sufficient supply for pandemic and inter-pandemic vaccines. Conventional dead virus vaccines are produced in embryonated eggs. Production in eggs is dependent on egg availability, recovery of approximately one dose per egg is quite low and the time between isolation of a new viral strain and a vaccine is quite long. Therefor we are developing alternative systems spanning from insect cell produced virus-like particles (VLPs) to plant expressed influenza antigens and alternative reverse genetic systems.


Project End 2010

  • Krammer, F., Pontiller, J., Tauer, C., Palmberger, D., Maccani, M., Baumann, M. and Grabherr, R.: Evaluation of the Influenza A Replicon for Transient Expression of Recombinant Proteins in Mammalian Cells. Plos One 2010
  • Krammer, F. and Grabherr, R.: Alternative influenza vaccines made by insect cells. Trends in Molecular Medicine, Review, 2010
  • Krammer , F. , Schinko, T., Messner, P., Palmberger, D., Ferko, B. and Grabherr, R.: Influenza virus-like particles as an antigen carrier platform for the presentation of the ESAT-6 epitope of Mycobacterium tuberculosis. Journal of Virological Methods 2010
  • Krammer, F., Schinko, T., Palmberger, D:, Tauer, C., Messner, P. and Grabherr, R.: Trichoplusia ni cells (High FiveTM) are highly efficient for the production of Influenza A virus-like particles; a comparison of two insect cell lines as production platforms for influenza vaccines.  Molecular Biotechnology 2010
  • Krammer, F., Nakowitsch, S., Messner, P., Palmberger, D., Ferko, B., Grabherr, R.: Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice. Biotechnol J 2010