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Juergen Mairhofer

  • Academic degree: Dipl.-Ing. Dr. / PhD
  • Date of birth: November 4th, 1980
  • Place of birth: Linz, Austria
  • Citizenship: Austria
  • Mail @
  • Publication details @Google Scholar

 


Education

  • 2011 – present Post Doctoral research fellow at the FFG Research Studios Austria / ProColi  
  • 2010  – 2011 Post Doctoral research fellow at the Austrian Centre of Industrial Biotechnology (ACIB), working in close collaboration with the Microbial Fermentation Group.
  • 2007 – 2010: PhD thesis "Innovations in Plasmid Production and Gene Therapy - Improved Safety, Productivity and Efficiency"
  • 2005 – 2006: Diploma thesis at the Baculo Virus Group of Prof. Reingard Grabherr, Institute of Applied Microbiology (IAM): “Improved antisense-constructs for targeted gene-silencing mediated by ColE1-type plasmid attendance - Towards the development of a novel antibiotic free plasmid selection system“
  • 1999 – 2006: University of Natural Resources and Applied Life Sciences Vienna:  Food- and Biotechnology
  • 1991 – 1999: Bundesrealgymnasium Rohrbach

 

GeneBank Entries

  • Escherichia coli K-12 RV308, complete genome LM995446
  • Escherichia coli K-12 HMS174, complete genome LM933812
  • Escherichia coli K-12 JM108 lon gene, GU117709
  • Escherichia coli BL21(DE3) complete genome, AM946981

Publications

  • Mairhofer J.*, Krempl PM., Thallinger G., Striedner G.: Finished Genome Sequence of the Laboratory Strain Escherichia coli HMS174 (ATCC 47011). GenomeA 2014, accepted for publication.
  • Krempl PM., Mairhofer J.*, Striedner G., Thallinger G.: Finished Genome Sequence of the Laboratory Strain Escherichia coli K-12 RV308 (ATCC 31608). GenomeA 2014, accepted for publication.
  • Mairhofer J.*, Wittwer A., Cserjan-Puschmann M., Striedner G.: Preventing T7 RNA polymerase read‐through transcription – a synthetic termination signal capable of improving bioprocess stability. ACS Synth Biol 2014, Epub ahead of print.
  • Oliveira P.H. and Mairhofer J.: Marker-free plasmids for biotechnological applications - implications and perspectives. Trends Biotechnol 2013, 31(9):539-47.
  • Jungreuthmayer C., Sonnleitner M., Striedner G., Mairhofer J., Zanghellini J.: Designing an optimally ethanol producing E. coli strain using constraint minimal cut sets. Proceedings of EUSIPCO 2013.  
  • Marisch M., Bayer K., Scharl T., Mairhofer J., Krempl P., Hummel K., Razzazi-Fazeli E., Striedner G.: A comparative analysis of industrial Escherichia coli K-12 and B strains in high-glucose batch cultivations on process-, transcriptome- and proteome level. PLOS one 2013.
  • Mairhofer J., Scharl T., Marisch K., Cserjan-Puschmann M., Striedner G.: Comparative transcription profiling and in-depth characterization of plasmid-based and plasmid-free Escherichia coli expression systems under production conditions. Applied Environ Microbiol 2013, 79(12):3802-12. 
  • Krempl P., Mairhofer J., Striedner G., Thallinger G.: A Sequence Comparison and Gene Expression Data Integration Add-on for the Pathway Tools Software. Bioinformatics 2012, Epub ahead of print.
  • Ribeiro S., Mairhofer J.*, Madeira C., Diogo M., Lobato da Silva C., Monteiro G., Grabherr R., Cabral J.: Plasmid DNA size does affect non-viral gene delivery efficiency in stem cells. Cell Reprogram 2012, 14(2):130-7.
  • Mairhofer J., Cserjan-Puschmann M., Striedner G., Nöbauer K., Razzazi-Fazeli E., Grabherr R.: Marker-free plasmids for gene therapeutic applications-Lack of antibiotic resistance gene substantially improves the manufacturing process. J Biotechnol 2010.
  • Mairhofer J., Roppert K., Ertl P.: Microfluidic Systems for Pathogen Sensing: A Review. Sensors 2009, 9, 4804-4823.
  • Mairhofer J., Grabherr R.: Rational vector design for efficient non-viral gene delivery: Challenges facing the use of plasmid DNA. Mol Biotech 2008, 39(2):97-104.
  • Mairhofer J., Pfaffenzeller I., Merz D., Grabherr R.: A novel antibiotic free plasmid selection system: Advances in safe and efficient DNA therapy. Biotechnol J  2008,3:83-89.
  • Pfaffenzeller I., Mairhofer J., Striedner G., Bayer K., Grabherr R.: Using ColE1-derived RNAI for supression of a bacterially encoded gene: Implication for a novel plasmid addiction system. Biotechnol J 2006, 1:675-681.

* = shared 1st authorship

Book chapters

Awards

Patents

Oral Presentations

  • Mairhofer J., Wittwer A., Cserjan-Puschmann M., Striedner G.: Small Changes, big difference - How a synthetic T7 transcriptional termination signal can improve bioprocess performance. 2nd Applied Synthetic Biology in Europe, Malaga, Spain, November 2013.
  • Mairhofer J., Marisch K., Scharl-Hirsch T., Striedner G.: Outer membrane composition of Escherichia coli does significantly influence overflow-metabolism and consequently acetate secretion. 7th Conference on Recombinant Protein Production (RPP7), Laupheim, Germany, March 2013.
  • Mairhofer J., Cserjan-Puschmann M., Striedner G., Grabherr R.: Marker‐free plasmids for gene therapeutic applications – A synthetic biology approach to prevent the usage of antibiotic resistance genes. 15th European Congress on Biotechnology, Istanbul, Turkey, September 2012.   
  • Mairhofer J., Cserjan-Puschmann M., Striedner G., Grabherr R.: Marker‐free plasmids for gene therapeutic applications – A synthetic biology approach to prevent the usage of antibiotics. Applied Synthetic Biology in Europe, Barcelona, Spain, February 2012.
  • Mairhofer J., Pfaffenzeller I., Merz D., Huber H., Grabherr R.: Antibiotic-free Selection System for the Manufacturing of Minimized Plasmids. 2nd International Workshop on Minicircle DNA, Bielefeld, Germany, May 2008.

Training

  • International Summer School on Advanced Techniques in Bacterial Genome Research,  28 Sept-3 Oct 2009, CeBiTec, University of Bielefeld.     
  • Systems Biology Short Course, June 8-12 2009, Center for Systems Biology, University of Iceland, Reykjavik.

Conference Contributions

  • Krempl P., Mairhofer J., Striedner G., Thallinger G.: A sequence comparison and gene expression data integration add-on for the Pathway Tools software., Conference on predicting Cell Metabolism and Phenotypes, Menlo Park, CA, USA, March 2013.
  • Cserjan-Puschman M., Török C., Luchner M.,  Mairhofer J., Striedner G. HTP mini-bioreactor system: screening platform for industrial recombinant protein production processes in E. coli  9 th European Symposium on Biochemical Engineering Science / 32 th International Symposium on the Separation of Proteins, Peptides and Polynucleotides. Istanbul, Turkey, September 2012.
  • Luchner M., Cserjan-Puschman M., Mairhofer J., Bayer K., Striedner G. Recombinant protein production with E. coli host and process design already be done or still under progress?  9 th European Symposium on Biochemical Engineering Science / 32 th International Symposium on the Separation of Proteins, Peptides and Polynucleotides. Istanbul, Turkey, September 2012.
  • Mairhofer J., Cserjan-Puschman M., Striedner G., Grabherr G. Antibiotic resistance-free plasmids for gene therapeutic applications - A synthetic biology approach to improve safety, yield and efficiency. International Conference on Systems Biology (ICSB), Toronto, Canada, August 2012. 
  • Mairhofer J., Cserjan-Puschman M., Smolar E., Luchner M., Bayer K., Grabherr G. and Striedner G. Integrated approach for the advanced characterization and improvement of T7-based Escherichia coli expression systems for recombinant protein production. Applied Synthetic Biology in Europe, Barcelona, Spain, February 2012. 
  • Mairhofer J., Cserjan-Puschman M., Smolar E., Luchner M., Bayer K., Grabherr G. and Striedner G. Integrated approach for the advanced characterization and improvement of T7-based Escherichia coli expression systems for recombinant protein production. 5th International Conference on Synthetic Biology (SB5.0), Stanford, CA, USA, June 2011. 
  • Mairhofer J., Ellersdorfer G., Sterflinger K., Grabherr R. Novelty  Seeking  -  Characterization  and  evaluation  of novel  environmental  microbial  strains  sampled  from extreme habitats. Workshop "Biorefinieries - White Biotechnology", Vienna, Austria, Oct. 2010. 
  • Mairhofer J., Strobl F., Striedner G., Grabherr R.: Improving DNA-based therapies: design of novel host, vectors and production strategies for the manufacturing of antibiotic marker-free plasmids. 14th European Congress on Biotechnology, Barcelona, Spain ,Sep. 2009.
  • Mairhofer J., Egger E., Pfaffenzeller I., Striedner G., Grabherr R.: Marker-Free Plasmid DNA Production - Advances in Safe and Efficient DNA Therapy. 5th Conference on Recombinant Protein Production (RPP), Alghero, Italy, Sep. 2008.
  • Mairhofer J., Pfaffenzeller I., Merz D., Grabherr R.: Minimal bacterial plasmids with improved transformation efficiencies. 8th Conference on Protein Expression in Animal Cells (PEACe), Angra Dos Reis, Brasil, Sep. 2007.
  • Keller D., Huber H., Mairhofer J., Pfaffenzeller I., Grabherr R.:  Development and Production of Marker-Free Non-Viral Vectors. 10th Annual Meeting of the American Society of Gene Therapy (ASGT), Seattle, USA, May 2007.
  • Mairhofer J., Pfaffenzeller I., Merz D., Grabherr R.: A novel platform for the antibiotic-free maintenance of therapeutic plasmids. 3rd International Conferance on DNA Vaccines, Malaga, Spain, May 2007.
  • Pfaffenzeller I., Mairhofer J., Striedner G., Bayer K., Grabherr R.: A novel approach for antibiotic free selection of recombinant plasmids for gene therapeutic applications based on RNA antisense reaction. Joint Annual Meeting of the ÖGBT, ÖGBM, ÖGGGT and ANGT, Salzburg, Austria, Sep. 2006.
  • Pfaffenzeller I., Mairhofer J., Striedner G., Bayer K., Grabherr R.: A novel approach for antibiotic-free selection of recombinant plasmids for gene therapeutic applications based on RNA antisense reaction. 7th Conference on Protein Expression in Animal Cells (PEACe), Hersonnisos, Crete, Greece. Sep 2005.
  • Mairhofer J., Pfaffenzeller I., Striedner G., Bayer K., Grabherr R.: Improved Antisense constructs for targeted gene-silencing mediated by ColE1-type plasmid attendance.    Joint Annual Meeting of the ÖGBT, ÖGBM, ÖGGGT and ANGT, Vienna. Sep. 2005.

Diploma Students: