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Stefan Heinl

  • Academic degree: Dipl.-Ing. Dr. nat. techn.
  • Citizenship: Austria
  • Mail @

Education

  • PhD, University of Natural Resources and Applied Life Sciences, Vienna, Austria (2008).
  • MSc of Foodscience and Biotechnology University of Natural Resources and Applied Life Sciences, Vienna, Austria (2004).

Employment History

  • 2008 to date: Senior scientist at the Christian Doppler Laboratory for Genetically Engineered Lactic Acid Bacteria (www.lactomics.at).
  • 2004 – 2008: PhD candidate at the Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
  • 2002 – 2004: Diploma thesis at theDepartment of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
  • 1999 – 2002: DHL International, Vienna, Austria.    

Internships

  • Hofbräu Kaltenhausen

  • Lebensmitteluntersuchungsanstalt der Stadt Wien

  • Milupa GmbH

  • Schweppes AG

Trainings

  • International Summer School on Advanced Techniques in Bacterial Genome Research, September, 28-October, 3, 2009 at the CeBiTec, Bielefeld.
  • Systems Biology Short Course, June 8-12 2009, Center for Systems Biology, University of Iceland, Reykjavik.

Publications

  • Heinl S., Spath K., Egger E., Grabherr R., 2011. Sequence analysis and characterization of two cryptic plasmids derived from Lactobacillus buchneri CD034. Plasmid. 66(3):159-68.
  • Spath K., Heinl S., Egger E.,  Grabherr R., 2011. Lactobacillus plantarum and Lactobacillus buchneri as Expression Systems: Evaluation of Different Origins of Replication for the Design of Suitable Shuttle Vectors. Molecular Biotechnology. DOI: 10.1007/s12033-011-9471-x
  • Hartinger, D., Heinl, S., Schwartz, H. E., Grabherr, R., Schatzmayr, G., Haltrich, D., Moll, W.-D., 2010. Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B(1). Microb Cell Fact. 9:62. doi: 10.1186/1475-2859-9-62.
  • Heinl, S., 2008. Identification, heterologous expression and functional analysis of genes involved in fumonisin detoxification. PhD thesis; University of Natural Resources and Applied Life Sciences, Vienna, Austria.
  • Heinl, S., 2004. Customised protein production for applications in diagnostics and vaccine development: expression and purification of human and HIV-proteins. Master thesis; University of Natural Resources and Applied Life Sciences, Vienna, Austria.

Oral presentations

  • Heinl, S., Spath, K., Egger, E., Gross, S. and Grabherr, R. Sequencing and characterization of two small cryptic plasmids derived from a Lactobacillus buchneri strain isolated from silage. 11th International Symposium on the Genetics of Industrial Microorganisms (GIM 2010), Melbourne, Australia, June 2010.

Patents

  • Moll, W-D., Hartinger, D., Heinl, S., Grießler, K., Binder, E. M., Schatzmayr, G., Grabherr, R. Method for the production of a feed additive for the oxygen-independent, enzymatic decomposition of mycotoxins, feed additive, and use thereof (WO/2010/031100).

Awards

  • WISP10, 3rd place.

Posters

  • Egger, E., Spath, K., Heinl, S., Gross, S., Grabherr, R. Identification of functional promoter sequences functional in different Lactobacillus strains. ÖGMBT Jahrestagung 2010, Wien.
  • Spath, K., Adamovich, N., Heinl, S., Grabherr, R. Transformation of industrial silage strains Lactobacillus buchneri and Lactobacillus reuteri. ÖGMBT Jahrestagung 2010, Wien.
  • Heinl, S., Spath, K., Egger, E., Gross, S., Schwab, H., Nussbaumer, S.,Grabherr, R., The Christian Doppler Laboratory for Genetically Engineered Lactic Acid Bacteria. ÖGMBT Jahrestagung 2010, Wien.
  • Heinl, S., Spath, K., Egger, E., Gross, S. and Grabherr, R. Sequencing and characterization of two small cryptic plasmids derived from a Lactobacillus buchneri strain isolated from silage. ESF-BU-CeBiTec Conference on Microbes and Industrial Biotechnology, Bielefeld, Germany, 21 – 24 November 2010
  • Egger, E., Heinl, S., Spath, K., Gross, S., Grabherr, R. Identification of strong promoters functional in different Lactobacillus strain. ESF-BU-CeBiTec Conference on Microbes and Industrial Biotechnology, Bielefeld, Germany, 21 – 24 November 2010.
  • Spath, K., Heinl, S., Egger, E., Grabherr, R. Break on through to the other side – electroporation of heavily transformable industrial lactic acid bacteria. ESF-BU-CeBiTec Conference on Microbes and Industrial Biotechnology, Bielefeld, Germany, 21 – 24 November 2010.
  • Egger E., Spath K., Heinl S., Gross S. and Grabherr R. Search for promoters and expression regulatory elements functional in lactic acid bacteria. 14th European Congress on Biotechnology, Barcelona, Spain , Sep. 2009. doi:10.1016/j.nbt.2009.06.686.
  • Spath K., Heinl S., Egger E., Grabherr, R.  Influence of different origins of replication on transformation efficiency, plasmid copy number and plasmid stability in an industrial lactic acid bacterial strain. 14th European Congress on Biotechnology, Barcelona, Spain , Sep. 2009. doi:10.1016/j.nbt.2009.06.289.
  • Heinl, S., Hartinger, D., Moll, W. D., Schatzmayr, G.  and Grabherr, R. Identification, heterologous expression and functional analysis of genes involved in the detoxification of fumonisin B1. 14th European Congress on Biotechnology, Barcelona, Spain , Sep. 2009.
  • Hartinger, D., Heinl, S.,  Grabherr, R., Schatzmayr, G.,  Moll, W.D. Heterologous expression and characterisation of a fumonisin-degrading enzyme. 5th Conference on Recombinant Protein Production (RPP), Alghero, Italy, Sep. 2008.
  • Heinl, S., Spath, K. and Grabherr, R. Vector-free cloning and heterologous expression of an endo-1,4-β-glucanase from Bacillus subtilis in Lactobacillus plantarum. 5th Conference on Recombinant Protein Production (RPP), Alghero, Italy, Sep. 2008.
  • Heinl, S., Gross, S., Ernst, W. and Grabherr, R.: Evaluation and optimisation of different cultivation systems for recombinant protein production in the baculovirus expression system. Joint Annual Meeting of the AAGGE,OEGM, OEGBT and ANGT, Innsbruck, Austria, Sep. 2004.
  • Heinl, S., Töllner, L., Ferko, B., Ernst, W. and Grabherr, R.  Generation of a polyclonal antiserum against Nef, a highly conserved regulatory protein of human immunodeficiency virus with the capacity of eliciting specific CTL response. Joint Annual Meeting of the nnual Meeting of the AAGGE,OEGM, OEGBT and ANGT, Graz, Austria, Sep. 2003.